Is viral RNA extraction necessary for amplifying genes from coronaviruses, like SARS-CoV-2?

NOT ANY MORE!

PCRopsis Reagent RVD allows you to perform direct RT-qPCR from nasopharyngeal / oropharyngeal samples transported in BD Universal Viral Transport media or Copan ESwab.

Simple Protocol:

  1. Mix sample 1:1 with Reagent RVD
  2. Heat to 95C for 5 minutes
  3. DONE… use ~5 uL into your RT-qPCR mix (can use 5 uL into a total 20 uL reaction)

Key Features:

  • Reagent RVD is an alternative to RNA extraction
  • Offers comparable RT-qPCR results to RNA extraction procedures for many applications in 5 MINUTES
  • Cost a fraction of traditional RNA extraction procedures, with fewer steps
  • Compatible with leading

“The U.S. Food and Drug Administration (FDA) reminds laboratory staff to use transport media (the liquid that maintains a specimen sample while it is transported to a laboratory) that are compatible with the SARS-CoV-2 testing platforms and the processes used in their laboratory to process samples collected from people who are being tested for SARS-CoV-2. There is a risk of exposure to harmful cyanide gas, a by-product of a reaction between guanidine thiocyanate or similar chemicals and bleach (sodium hypochlorite), when certain transport media are used with an incompatible testing platform or laboratory process. Guanidine thiocyanate may be referred to

An Elephant, a Dog and an Ass,
Tried to see who would pee the fastest,
On the referee’s go,
All three unleashed their flow,
And learnt: Pee time is constant over mass*.

*assuming mass > 3 kg The first sentence of this paper reads: “We filmed the urination of 16 animals and obtained 28 videos of urination from YouTube, listed in SI Appendix. Movies S1–S4 show that urination style is strongly dependent on animal size.” http://www.pnas.org/content/111/33/11932.full

compoundchem:

It’s often said that cooking is like chemistry, or vice versa. So here’s a look at some of the organic compounds you can find in various herbs and spices. Whilst they are all a complex mixture of various organic compounds, this highlights those that contribute significantly to their taste, flavour or aroma.

You can read more about each of the compounds here: http://wp.me/p4aPLT-8c