Category Archives: Technology

OpsisDx highlighted in peer-reviewed journal

The novel scientific concept behind OpsisDx™, developed by Entopsis Inc., is outlined in a recently published peer-reviewed scientific manuscript in the Royal Society of Chemistry Analyst journal. In addition, OpsisDx™ was selected by the editors of the journal to be featured on the journal’s cover.

The manuscript, entitled ‘A colorimetric chemical tongue detects and distinguishes between
multiple analytes’, demonstrates how photoinitiators can be used to profile simple and complex chemical mixtures using an array of polymers with distinct binding properties. The OpsisDx™ research team is exploring whether this approach can can be the basis for a multi-disease detection platform that may

PCRopsis LLC launches Oral Rinse: a unique device for the collection and transportation of oral samples with distinct advantages

Oral samples offer a simple, low cost, and user-friendly means of collecting oral samples for a variety of applications; such as, DNA sequencing, respiratory virus testing, oral cancer detection, toxicology, and many others. These samples can be conveniently collected at home and shipped to testing sites.

Key Advantages of PCRopsis™ Oral Rinse over Alternative Approaches:

1. Simple sample collection: safe ingredients allow for self collection of oral samples by swishing the liquid in your mouth – no need for swabs, secondary inactivating reagents, careful mixing of saliva with reagents, or pipettes to transfer small volumes
2. Keeps RNA stable at room

PCRopsis™ RVD Enhancer makes PCRopsis™ Reagent RVD compatible with most specimen transport mediums

PCRopsis™ Reagent RVD + PCRopsis™ RVD Enhancer offers a direct PCR solution for specimens in most non-inactivating transport mediums.

PCRopsis™ Reagent RVD alone has been repeatedly shown to facilitate extraction-free amplification of viral, bacterial, and mammalian specimens in universal viral transport (UVT) mediums, like BD™ Universal Viral Transport system. This is one of the most common specimen transport mediums in the U.S., although many other transport mediums are used in the U.S. and abroad.

Entopsis developed PCRopsis™ RVD Enhancer to expand the compatibility of PCRopsis™ Reagent RVD to process specimens in diverse transport mediums. PCRopsis™ Reagent RVD, when used in

Beyond COVID-19 Testing: Reagent RVD Facilitates Extraction-free Amplification of Bacteria

Reagent RVD has proven capable of amplifying SARS-CoV-2 gene targets directly from universal viral transport (UVT) medium without the need for viral RNA extraction. However, diagnostic reference laboratories also want to use this approach for the amplification of bacterial gene targets.

The PCRopsis™ research team has extended their validation efforts in this direction. It’s been confirmed that Reagent RVD is capable of mediating extraction-free qPCR of specimens containing gram positive and negative bacteria.

To date, Reagent RVD facilitates extraction-free amplification of viral, bacterial and human gene targets directly from UVT.

This opens the door to new applications with Reagent

Non-invasive OpsisDx Diagnostic Test Awarded US Academy of Medicine Research Global Award

The U.S. National Academy of Medicine’s Healthy Longevity Global Grand Challenge has awarded a grant to develop Entopsis’ OpsisDx(TM) platform for detecting Parkinson’s disease using skin swabs or urine. This effort is driven by Entopsis’ scientific and clinical collaborators at Singapore’s Temasek Life Sciences Laboratory (TLL) and National Neuroscience Institute (NNI).

Read more about it here:

https://www.einnews.com/pr_news/529273833/national-academy-of-medicine-selects-opsisdx-for-co-development-of-a-urine-based-diagnostic-for-parkinson-s-disease

Non-invasive OpsisDx Diagnostic Test Awarded US Academy Research Grant

Entopsis Partners with Madison Core Labs: PCRopsis Reagent RVD

Our team’s passion is to innovate and create powerful, unheard-of solutions to the world’s problems. We pride ourselves on our creativity and desire to make a meaningful difference, both socially and scientifically.

However, we can only achieve our global goals with the right partners that share our mission. Today’s press release (see below) highlights one such partner, Madison Core Labs.

Link to Press Release

Madison Core Laboratories Initiates New Innovation Effort to Better Serve Clients

August 13, 2020 09:05 AM Eastern Daylight Time

HUNTSVILLE, Ala.–(BUSINESS WIRE)–

PCRopsis Reagent RVD Launched!

Is viral RNA extraction necessary for amplifying genes from coronaviruses, like SARS-CoV-2?

NOT ANY MORE!

PCRopsis Reagent RVD allows you to perform direct RT-qPCR from nasopharyngeal / oropharyngeal samples transported in BD Universal Viral Transport media or Copan ESwab.

Simple Protocol:

  1. Mix sample 1:1 with Reagent RVD
  2. Heat to 95C for 10 minutes
  3. DONE… use ~5 uL into your RT-qPCR mix (can use 5 uL into a total 20 uL reaction)

Key Features:

  • Reagent RVD is an alternative to RNA extraction
  • Offers comparable RT-qPCR results to RNA extraction procedures for many applications in 10 MINUTES
  • Cost a fraction of traditional RNA extraction procedures, with fewer steps
  • Compatible

Looking for warriors (i.e., Entopsis team members)

1) CLIA Lab Director (Ph.D. with LDT development experience)
Location: Orlando, FL, USA

2) CLIA Medical Technologist (management experience needed)
Location: Orlando, FL, USA

3) Computer Scientist – Machine Learning
Location: Singapore, Singapore

4) Online Sales / Marketing
Location: global (all work is online)

5) Personal / Business Assistant to CEO (…a jack of all trades)
Location: Miami, FL, USA

6) Content Writer (little work experience necessary, but must be a daring writer)
Location: Miami, FL, USA

info@entopsis.com

Gen 5 NuTec Slides Finalized

Our team of multi-disciplinary scientists and engineers have once again improved on the NuTec Slide. This was only made possible by building on past insights and proprietary know-how. The latest version of NuTec Slides show improved reproducibility and sample discernment, while being able to handle a wider range of sample intensities. The Gen 5 NuTec Slide will be used for commercial applications in the U.S.

PCRopsis Technology to Combat COVID-19

A shortage of COVID-19 testing supplies is hampering people’s ability to perform timely RT-PCR testing. A key bottleneck is viral RNA extraction, which involves multiple steps, reagents and devices.

Our team is now investigating whether we can apply our PCRopsis technology and know-how to facilitate direct RT-PCR from nasal-pharyngeal and saliva samples by skipping the RNA extraction step.

Stay tuned for research updates on this promising approach in the battle against the pandemic.

Check-out our other PCRopsis products that allow you to perform PCR directly from clinical samples: PCRopsis.com

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